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mouse il-1β high sensitivity elisa kit  (Multi Sciences (Lianke) Biotech Co Ltd)


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    Structured Review

    Multi Sciences (Lianke) Biotech Co Ltd mouse il-1β high sensitivity elisa kit
    Mouse Il 1β High Sensitivity Elisa Kit, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 95/100, based on 844 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+il/Mouse+IL-1%CE%B2+High+Sensitivity+ELISA+Kit/custom%40ek201bhs%4042692281
    Average 95 stars, based on 844 article reviews
    mouse il-1β high sensitivity elisa kit - by Bioz Stars, 2026-09
    95/100 stars

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    Liposomes:

    Article Title: Commensal enteric virome regulates intestinal carbohydrate digestion and absorption.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CD45 Rabbit PolymAb ABclonal Cat#A24802PM CD3E Rabbit mAb ABclonal Cat#A19017 APC Rat Anti-Mouse CD8a(53–6.7) BD Pharmingen Cat#561093 PE-Cy7 Rat anti-Mouse CD4 Antibody(GK1.5) Absin Cat#abs1850076 PE-Cy7 Rat Anti-Mouse CD127(SB/199) BD Pharmingen Cat#560733 Alexa Fluor 647 anti-mouse IL-22 Biolegend Cat#516406 FITC Rat Anti-Mouse CD45 BD Pharmingen Cat#561088 PE Rat Anti-Mouse IL-17A BD Pharmingen Cat#561020 PerCP-CyTM5.5 Mouse Anti-Human CD3 BD Pharmingen Cat#560835 PerCP-CyTM5.5 Mouse Lineage Antibody Cocktail BD Pharmingen Cat#561317 PE Rabbit anti-Mouse T-bet/Tbx21 mAb ABclonal Cat#A28217 PE-CyTM7 Rat Anti-Mouse IFN-γ BD Pharmingen Cat#557649 SGLT1 Polyclonal antibody Proteintech Cat#30861-1-AP GLUT2 Polyclonal antibody Proteintech Cat#20436-1-AP InVivoMAb anti-mouse IL-23 (p19) BioXcell Cat#BE0313 IL-22 Monoclonal Antibody (IL22JOP) ThermoFisher Cat#16-7222-82 InVivoMAb anti-mouse MHC Class II (I-A/I-E) BioXcell Cat#BE0108 InVivoMAb anti-mouse CD4 (Clone: GK1.5) BioXcell Cat#BE0003-41 InVivoMAb anti-mouse CD8α (Clone: 53–6.7) BioXcell Cat#BE0004-1 APC Rabbit anti-Mouse F4/80 mAb ABclonal Cat#A28171 Bacterial and virus strains Streptococcus salivarius BNCC Cat#BNCC134928 Staphylococcus epidermidis BNCC Cat#BNCC102555 Pseudomonas aeruginosa BNCC Cat#BNCC340634 Clostridium butyricum BNCC Cat#BNCC337239 Escherichia coli C-3000 HZBMC Cat#HZB364542 Escherichia phage T7 BIOBW Cat#bio-108775 Primary enteric bacteriophages This study Table S2 Chemicals, peptides, and recombinant proteins Mouse IL-22 Recombinant Protein ThermoFisher Cat#210-22 IFN-lambda 3/IL-28B Protein, Mouse (HEK293, His) MedChemExpress Cat#HY-P74851 IL-17A Protein, Mouse (CHO) MedChemExpress Cat#HY-P7372 Acriflavine Aladdin Cat#A103028 Lamivudine Aladdin Cat#L408170 Aciclovir Aladdin Cat#A126073 Ribavirin Aladdin Cat#R101754 Ampicillin Aladdin Cat#A433389 Vancomycin Aladdin Cat#V301569 Neomycin sulfate Aladdin Cat#N109017 Metronidazole Aladdin Cat#M408417 Amphotericin B Aladdin Cat#A408132 PEG 8000 Sangon Biotech Cat#A100159 SYBR Gold nucleic acid gel stain ThermoFisher Cat#S11494 (Continued on next page) Cell Host & Microbe 34, 1–16.e1–e8, March 11, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER LB Broth hopebio Cat#HB0128 Nutrient Broth hopebio Cat#HB0108 Collagenase type 3 Worthington Cat#LS004182 Percoll Sigma Cat#P7828 Clodronate liposomes Yeasen Cat#40337ES08 Trizol ThermoFisher Cat#15596018CN DNase Sigma Cat#DN-25 Phenol/Chloroform/Isoamyl alcohol (25:24:1) Sigma Cat#P3803 Phusion DNA polymerase New England Biolabs Cat#M0530S 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester Aladdin Cat#C107884 Lipofectamine 2000 ThermoFisher Cat#11668030 Critical commercial assays Periodic Acid-Schiff Staining Kit Beyotime Cat#C0142S Pan T Cell Isolation Kit II, mouse Miltenyi Biotec Cat#130-095-130 Anti-F4/80 MicroBeads UltraPure, mouse Miltenyi Biotec Cat#130-110-443 EasySep Mouse Pan-ILC Enrichment Kit STEMCELL Technologies Cat#19875 Mouse IL-22 High Sensitivity ELISA Kit Multi sciences Cat#EK222HS Mouse IL-23 ELISA Kit Multi sciences CatEK223 Mouse Insulin ELISA Kit MEIMIAN Cat#MM-0579M2 Mouse IL-6 ELISA Kit RUISAIQI Cat#R-S-M0007 Mouse IL-1β ELISA Kit RUISAIQI Cat#R-S-M0003 Mouse TNF-α ELISA Kit RUISAIQI Cat#R-S-M0002 Mouse Adiponectin ELISA Kit RUISAIQI Cat#R-S-M10108 Evo M-MLV RT Kit with gDNA Clean for qPCR Accurate biology Cat#AG11728 TB Green Premix Ex Taq II (Tli RNase H Plus) Takara Cat#RR820Q E.Z.N.A. .. DNA Kit Omega Bio-tek Cat#D3892-01 TruSeq Nano DNA sample preparation Kit Illumina Cat#15041877 QIAamp MinElute Virus Spin Kit Qiagen Cat#57704 QIAquick Gel Extraction Kit Qiagen Cat#28706 TruSeq RNA Library Prep Kit Illumina Cat#RS-122-2001 Ribo-Zero rRNA Removal Kit Epicenter Cat#15066012 BCA Protein Assay Kit ThermoFisher Cat#223250 quick-dna fecal/soil microbe miniprep kit Zymo research Cat#D6010S Deposited data Enteric virome and bacteriome sequencing, and mouse small intestinal scRNA-seq and bulk RNA sequencing This study PRJCA029200 https://ngdc.cncb.ac.cn/gsa/ Experimental models: Cell lines Caco-2 ATCC Cat#HTB-37 Experimental models: Organisms/strains BALB/cNj-Foxn1nu/Gpt GemPharmatech D000521 RRID:IMSR_GPT:D000521 C57BL/6JGpt GemPharmatech N000013 RRID:IMSR_GPT:N000013 BALB/cJGpt GemPharmatech N000020 RRID:IMSR_GPT:N000020 C57BL/6JGpt-Rag2em6Cd6972/Gpt GemPharmatech T011534 RRID:IMSR_GPT:T011534 C57BL/6JGpt-Il22em4Cd16159/Gpt GemPharmatech T052618 RRID:IMSR_GPT:T052618 C57BL/6Smoc-Cd74em1Smoc Shanghai Model Organisms NM-KO-190772 RRID: IMSR_NM-KO-190772 Oligonucleotides Primers for qPCR, see Table S3 Sangon Biotech N/A (Continued on next page) e2 Cell Host & Microbe 34, 1–16.e1–e8, March 11, 2026

    Staining:

    Article Title: Commensal enteric virome regulates intestinal carbohydrate digestion and absorption.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CD45 Rabbit PolymAb ABclonal Cat#A24802PM CD3E Rabbit mAb ABclonal Cat#A19017 APC Rat Anti-Mouse CD8a(53–6.7) BD Pharmingen Cat#561093 PE-Cy7 Rat anti-Mouse CD4 Antibody(GK1.5) Absin Cat#abs1850076 PE-Cy7 Rat Anti-Mouse CD127(SB/199) BD Pharmingen Cat#560733 Alexa Fluor 647 anti-mouse IL-22 Biolegend Cat#516406 FITC Rat Anti-Mouse CD45 BD Pharmingen Cat#561088 PE Rat Anti-Mouse IL-17A BD Pharmingen Cat#561020 PerCP-CyTM5.5 Mouse Anti-Human CD3 BD Pharmingen Cat#560835 PerCP-CyTM5.5 Mouse Lineage Antibody Cocktail BD Pharmingen Cat#561317 PE Rabbit anti-Mouse T-bet/Tbx21 mAb ABclonal Cat#A28217 PE-CyTM7 Rat Anti-Mouse IFN-γ BD Pharmingen Cat#557649 SGLT1 Polyclonal antibody Proteintech Cat#30861-1-AP GLUT2 Polyclonal antibody Proteintech Cat#20436-1-AP InVivoMAb anti-mouse IL-23 (p19) BioXcell Cat#BE0313 IL-22 Monoclonal Antibody (IL22JOP) ThermoFisher Cat#16-7222-82 InVivoMAb anti-mouse MHC Class II (I-A/I-E) BioXcell Cat#BE0108 InVivoMAb anti-mouse CD4 (Clone: GK1.5) BioXcell Cat#BE0003-41 InVivoMAb anti-mouse CD8α (Clone: 53–6.7) BioXcell Cat#BE0004-1 APC Rabbit anti-Mouse F4/80 mAb ABclonal Cat#A28171 Bacterial and virus strains Streptococcus salivarius BNCC Cat#BNCC134928 Staphylococcus epidermidis BNCC Cat#BNCC102555 Pseudomonas aeruginosa BNCC Cat#BNCC340634 Clostridium butyricum BNCC Cat#BNCC337239 Escherichia coli C-3000 HZBMC Cat#HZB364542 Escherichia phage T7 BIOBW Cat#bio-108775 Primary enteric bacteriophages This study Table S2 Chemicals, peptides, and recombinant proteins Mouse IL-22 Recombinant Protein ThermoFisher Cat#210-22 IFN-lambda 3/IL-28B Protein, Mouse (HEK293, His) MedChemExpress Cat#HY-P74851 IL-17A Protein, Mouse (CHO) MedChemExpress Cat#HY-P7372 Acriflavine Aladdin Cat#A103028 Lamivudine Aladdin Cat#L408170 Aciclovir Aladdin Cat#A126073 Ribavirin Aladdin Cat#R101754 Ampicillin Aladdin Cat#A433389 Vancomycin Aladdin Cat#V301569 Neomycin sulfate Aladdin Cat#N109017 Metronidazole Aladdin Cat#M408417 Amphotericin B Aladdin Cat#A408132 PEG 8000 Sangon Biotech Cat#A100159 SYBR Gold nucleic acid gel stain ThermoFisher Cat#S11494 (Continued on next page) Cell Host & Microbe 34, 1–16.e1–e8, March 11, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER LB Broth hopebio Cat#HB0128 Nutrient Broth hopebio Cat#HB0108 Collagenase type 3 Worthington Cat#LS004182 Percoll Sigma Cat#P7828 Clodronate liposomes Yeasen Cat#40337ES08 Trizol ThermoFisher Cat#15596018CN DNase Sigma Cat#DN-25 Phenol/Chloroform/Isoamyl alcohol (25:24:1) Sigma Cat#P3803 Phusion DNA polymerase New England Biolabs Cat#M0530S 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester Aladdin Cat#C107884 Lipofectamine 2000 ThermoFisher Cat#11668030 Critical commercial assays Periodic Acid-Schiff Staining Kit Beyotime Cat#C0142S Pan T Cell Isolation Kit II, mouse Miltenyi Biotec Cat#130-095-130 Anti-F4/80 MicroBeads UltraPure, mouse Miltenyi Biotec Cat#130-110-443 EasySep Mouse Pan-ILC Enrichment Kit STEMCELL Technologies Cat#19875 Mouse IL-22 High Sensitivity ELISA Kit Multi sciences Cat#EK222HS Mouse IL-23 ELISA Kit Multi sciences CatEK223 Mouse Insulin ELISA Kit MEIMIAN Cat#MM-0579M2 Mouse IL-6 ELISA Kit RUISAIQI Cat#R-S-M0007 Mouse IL-1β ELISA Kit RUISAIQI Cat#R-S-M0003 Mouse TNF-α ELISA Kit RUISAIQI Cat#R-S-M0002 Mouse Adiponectin ELISA Kit RUISAIQI Cat#R-S-M10108 Evo M-MLV RT Kit with gDNA Clean for qPCR Accurate biology Cat#AG11728 TB Green Premix Ex Taq II (Tli RNase H Plus) Takara Cat#RR820Q E.Z.N.A. .. DNA Kit Omega Bio-tek Cat#D3892-01 TruSeq Nano DNA sample preparation Kit Illumina Cat#15041877 QIAamp MinElute Virus Spin Kit Qiagen Cat#57704 QIAquick Gel Extraction Kit Qiagen Cat#28706 TruSeq RNA Library Prep Kit Illumina Cat#RS-122-2001 Ribo-Zero rRNA Removal Kit Epicenter Cat#15066012 BCA Protein Assay Kit ThermoFisher Cat#223250 quick-dna fecal/soil microbe miniprep kit Zymo research Cat#D6010S Deposited data Enteric virome and bacteriome sequencing, and mouse small intestinal scRNA-seq and bulk RNA sequencing This study PRJCA029200 https://ngdc.cncb.ac.cn/gsa/ Experimental models: Cell lines Caco-2 ATCC Cat#HTB-37 Experimental models: Organisms/strains BALB/cNj-Foxn1nu/Gpt GemPharmatech D000521 RRID:IMSR_GPT:D000521 C57BL/6JGpt GemPharmatech N000013 RRID:IMSR_GPT:N000013 BALB/cJGpt GemPharmatech N000020 RRID:IMSR_GPT:N000020 C57BL/6JGpt-Rag2em6Cd6972/Gpt GemPharmatech T011534 RRID:IMSR_GPT:T011534 C57BL/6JGpt-Il22em4Cd16159/Gpt GemPharmatech T052618 RRID:IMSR_GPT:T052618 C57BL/6Smoc-Cd74em1Smoc Shanghai Model Organisms NM-KO-190772 RRID: IMSR_NM-KO-190772 Oligonucleotides Primers for qPCR, see Table S3 Sangon Biotech N/A (Continued on next page) e2 Cell Host & Microbe 34, 1–16.e1–e8, March 11, 2026

    Cell Isolation:

    Article Title: Commensal enteric virome regulates intestinal carbohydrate digestion and absorption.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CD45 Rabbit PolymAb ABclonal Cat#A24802PM CD3E Rabbit mAb ABclonal Cat#A19017 APC Rat Anti-Mouse CD8a(53–6.7) BD Pharmingen Cat#561093 PE-Cy7 Rat anti-Mouse CD4 Antibody(GK1.5) Absin Cat#abs1850076 PE-Cy7 Rat Anti-Mouse CD127(SB/199) BD Pharmingen Cat#560733 Alexa Fluor 647 anti-mouse IL-22 Biolegend Cat#516406 FITC Rat Anti-Mouse CD45 BD Pharmingen Cat#561088 PE Rat Anti-Mouse IL-17A BD Pharmingen Cat#561020 PerCP-CyTM5.5 Mouse Anti-Human CD3 BD Pharmingen Cat#560835 PerCP-CyTM5.5 Mouse Lineage Antibody Cocktail BD Pharmingen Cat#561317 PE Rabbit anti-Mouse T-bet/Tbx21 mAb ABclonal Cat#A28217 PE-CyTM7 Rat Anti-Mouse IFN-γ BD Pharmingen Cat#557649 SGLT1 Polyclonal antibody Proteintech Cat#30861-1-AP GLUT2 Polyclonal antibody Proteintech Cat#20436-1-AP InVivoMAb anti-mouse IL-23 (p19) BioXcell Cat#BE0313 IL-22 Monoclonal Antibody (IL22JOP) ThermoFisher Cat#16-7222-82 InVivoMAb anti-mouse MHC Class II (I-A/I-E) BioXcell Cat#BE0108 InVivoMAb anti-mouse CD4 (Clone: GK1.5) BioXcell Cat#BE0003-41 InVivoMAb anti-mouse CD8α (Clone: 53–6.7) BioXcell Cat#BE0004-1 APC Rabbit anti-Mouse F4/80 mAb ABclonal Cat#A28171 Bacterial and virus strains Streptococcus salivarius BNCC Cat#BNCC134928 Staphylococcus epidermidis BNCC Cat#BNCC102555 Pseudomonas aeruginosa BNCC Cat#BNCC340634 Clostridium butyricum BNCC Cat#BNCC337239 Escherichia coli C-3000 HZBMC Cat#HZB364542 Escherichia phage T7 BIOBW Cat#bio-108775 Primary enteric bacteriophages This study Table S2 Chemicals, peptides, and recombinant proteins Mouse IL-22 Recombinant Protein ThermoFisher Cat#210-22 IFN-lambda 3/IL-28B Protein, Mouse (HEK293, His) MedChemExpress Cat#HY-P74851 IL-17A Protein, Mouse (CHO) MedChemExpress Cat#HY-P7372 Acriflavine Aladdin Cat#A103028 Lamivudine Aladdin Cat#L408170 Aciclovir Aladdin Cat#A126073 Ribavirin Aladdin Cat#R101754 Ampicillin Aladdin Cat#A433389 Vancomycin Aladdin Cat#V301569 Neomycin sulfate Aladdin Cat#N109017 Metronidazole Aladdin Cat#M408417 Amphotericin B Aladdin Cat#A408132 PEG 8000 Sangon Biotech Cat#A100159 SYBR Gold nucleic acid gel stain ThermoFisher Cat#S11494 (Continued on next page) Cell Host & Microbe 34, 1–16.e1–e8, March 11, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER LB Broth hopebio Cat#HB0128 Nutrient Broth hopebio Cat#HB0108 Collagenase type 3 Worthington Cat#LS004182 Percoll Sigma Cat#P7828 Clodronate liposomes Yeasen Cat#40337ES08 Trizol ThermoFisher Cat#15596018CN DNase Sigma Cat#DN-25 Phenol/Chloroform/Isoamyl alcohol (25:24:1) Sigma Cat#P3803 Phusion DNA polymerase New England Biolabs Cat#M0530S 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester Aladdin Cat#C107884 Lipofectamine 2000 ThermoFisher Cat#11668030 Critical commercial assays Periodic Acid-Schiff Staining Kit Beyotime Cat#C0142S Pan T Cell Isolation Kit II, mouse Miltenyi Biotec Cat#130-095-130 Anti-F4/80 MicroBeads UltraPure, mouse Miltenyi Biotec Cat#130-110-443 EasySep Mouse Pan-ILC Enrichment Kit STEMCELL Technologies Cat#19875 Mouse IL-22 High Sensitivity ELISA Kit Multi sciences Cat#EK222HS Mouse IL-23 ELISA Kit Multi sciences CatEK223 Mouse Insulin ELISA Kit MEIMIAN Cat#MM-0579M2 Mouse IL-6 ELISA Kit RUISAIQI Cat#R-S-M0007 Mouse IL-1β ELISA Kit RUISAIQI Cat#R-S-M0003 Mouse TNF-α ELISA Kit RUISAIQI Cat#R-S-M0002 Mouse Adiponectin ELISA Kit RUISAIQI Cat#R-S-M10108 Evo M-MLV RT Kit with gDNA Clean for qPCR Accurate biology Cat#AG11728 TB Green Premix Ex Taq II (Tli RNase H Plus) Takara Cat#RR820Q E.Z.N.A. .. DNA Kit Omega Bio-tek Cat#D3892-01 TruSeq Nano DNA sample preparation Kit Illumina Cat#15041877 QIAamp MinElute Virus Spin Kit Qiagen Cat#57704 QIAquick Gel Extraction Kit Qiagen Cat#28706 TruSeq RNA Library Prep Kit Illumina Cat#RS-122-2001 Ribo-Zero rRNA Removal Kit Epicenter Cat#15066012 BCA Protein Assay Kit ThermoFisher Cat#223250 quick-dna fecal/soil microbe miniprep kit Zymo research Cat#D6010S Deposited data Enteric virome and bacteriome sequencing, and mouse small intestinal scRNA-seq and bulk RNA sequencing This study PRJCA029200 https://ngdc.cncb.ac.cn/gsa/ Experimental models: Cell lines Caco-2 ATCC Cat#HTB-37 Experimental models: Organisms/strains BALB/cNj-Foxn1nu/Gpt GemPharmatech D000521 RRID:IMSR_GPT:D000521 C57BL/6JGpt GemPharmatech N000013 RRID:IMSR_GPT:N000013 BALB/cJGpt GemPharmatech N000020 RRID:IMSR_GPT:N000020 C57BL/6JGpt-Rag2em6Cd6972/Gpt GemPharmatech T011534 RRID:IMSR_GPT:T011534 C57BL/6JGpt-Il22em4Cd16159/Gpt GemPharmatech T052618 RRID:IMSR_GPT:T052618 C57BL/6Smoc-Cd74em1Smoc Shanghai Model Organisms NM-KO-190772 RRID: IMSR_NM-KO-190772 Oligonucleotides Primers for qPCR, see Table S3 Sangon Biotech N/A (Continued on next page) e2 Cell Host & Microbe 34, 1–16.e1–e8, March 11, 2026

    Enzyme-linked Immunosorbent Assay:

    Article Title: Commensal enteric virome regulates intestinal carbohydrate digestion and absorption.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CD45 Rabbit PolymAb ABclonal Cat#A24802PM CD3E Rabbit mAb ABclonal Cat#A19017 APC Rat Anti-Mouse CD8a(53–6.7) BD Pharmingen Cat#561093 PE-Cy7 Rat anti-Mouse CD4 Antibody(GK1.5) Absin Cat#abs1850076 PE-Cy7 Rat Anti-Mouse CD127(SB/199) BD Pharmingen Cat#560733 Alexa Fluor 647 anti-mouse IL-22 Biolegend Cat#516406 FITC Rat Anti-Mouse CD45 BD Pharmingen Cat#561088 PE Rat Anti-Mouse IL-17A BD Pharmingen Cat#561020 PerCP-CyTM5.5 Mouse Anti-Human CD3 BD Pharmingen Cat#560835 PerCP-CyTM5.5 Mouse Lineage Antibody Cocktail BD Pharmingen Cat#561317 PE Rabbit anti-Mouse T-bet/Tbx21 mAb ABclonal Cat#A28217 PE-CyTM7 Rat Anti-Mouse IFN-γ BD Pharmingen Cat#557649 SGLT1 Polyclonal antibody Proteintech Cat#30861-1-AP GLUT2 Polyclonal antibody Proteintech Cat#20436-1-AP InVivoMAb anti-mouse IL-23 (p19) BioXcell Cat#BE0313 IL-22 Monoclonal Antibody (IL22JOP) ThermoFisher Cat#16-7222-82 InVivoMAb anti-mouse MHC Class II (I-A/I-E) BioXcell Cat#BE0108 InVivoMAb anti-mouse CD4 (Clone: GK1.5) BioXcell Cat#BE0003-41 InVivoMAb anti-mouse CD8α (Clone: 53–6.7) BioXcell Cat#BE0004-1 APC Rabbit anti-Mouse F4/80 mAb ABclonal Cat#A28171 Bacterial and virus strains Streptococcus salivarius BNCC Cat#BNCC134928 Staphylococcus epidermidis BNCC Cat#BNCC102555 Pseudomonas aeruginosa BNCC Cat#BNCC340634 Clostridium butyricum BNCC Cat#BNCC337239 Escherichia coli C-3000 HZBMC Cat#HZB364542 Escherichia phage T7 BIOBW Cat#bio-108775 Primary enteric bacteriophages This study Table S2 Chemicals, peptides, and recombinant proteins Mouse IL-22 Recombinant Protein ThermoFisher Cat#210-22 IFN-lambda 3/IL-28B Protein, Mouse (HEK293, His) MedChemExpress Cat#HY-P74851 IL-17A Protein, Mouse (CHO) MedChemExpress Cat#HY-P7372 Acriflavine Aladdin Cat#A103028 Lamivudine Aladdin Cat#L408170 Aciclovir Aladdin Cat#A126073 Ribavirin Aladdin Cat#R101754 Ampicillin Aladdin Cat#A433389 Vancomycin Aladdin Cat#V301569 Neomycin sulfate Aladdin Cat#N109017 Metronidazole Aladdin Cat#M408417 Amphotericin B Aladdin Cat#A408132 PEG 8000 Sangon Biotech Cat#A100159 SYBR Gold nucleic acid gel stain ThermoFisher Cat#S11494 (Continued on next page) Cell Host & Microbe 34, 1–16.e1–e8, March 11, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER LB Broth hopebio Cat#HB0128 Nutrient Broth hopebio Cat#HB0108 Collagenase type 3 Worthington Cat#LS004182 Percoll Sigma Cat#P7828 Clodronate liposomes Yeasen Cat#40337ES08 Trizol ThermoFisher Cat#15596018CN DNase Sigma Cat#DN-25 Phenol/Chloroform/Isoamyl alcohol (25:24:1) Sigma Cat#P3803 Phusion DNA polymerase New England Biolabs Cat#M0530S 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester Aladdin Cat#C107884 Lipofectamine 2000 ThermoFisher Cat#11668030 Critical commercial assays Periodic Acid-Schiff Staining Kit Beyotime Cat#C0142S Pan T Cell Isolation Kit II, mouse Miltenyi Biotec Cat#130-095-130 Anti-F4/80 MicroBeads UltraPure, mouse Miltenyi Biotec Cat#130-110-443 EasySep Mouse Pan-ILC Enrichment Kit STEMCELL Technologies Cat#19875 Mouse IL-22 High Sensitivity ELISA Kit Multi sciences Cat#EK222HS Mouse IL-23 ELISA Kit Multi sciences CatEK223 Mouse Insulin ELISA Kit MEIMIAN Cat#MM-0579M2 Mouse IL-6 ELISA Kit RUISAIQI Cat#R-S-M0007 Mouse IL-1β ELISA Kit RUISAIQI Cat#R-S-M0003 Mouse TNF-α ELISA Kit RUISAIQI Cat#R-S-M0002 Mouse Adiponectin ELISA Kit RUISAIQI Cat#R-S-M10108 Evo M-MLV RT Kit with gDNA Clean for qPCR Accurate biology Cat#AG11728 TB Green Premix Ex Taq II (Tli RNase H Plus) Takara Cat#RR820Q E.Z.N.A. .. DNA Kit Omega Bio-tek Cat#D3892-01 TruSeq Nano DNA sample preparation Kit Illumina Cat#15041877 QIAamp MinElute Virus Spin Kit Qiagen Cat#57704 QIAquick Gel Extraction Kit Qiagen Cat#28706 TruSeq RNA Library Prep Kit Illumina Cat#RS-122-2001 Ribo-Zero rRNA Removal Kit Epicenter Cat#15066012 BCA Protein Assay Kit ThermoFisher Cat#223250 quick-dna fecal/soil microbe miniprep kit Zymo research Cat#D6010S Deposited data Enteric virome and bacteriome sequencing, and mouse small intestinal scRNA-seq and bulk RNA sequencing This study PRJCA029200 https://ngdc.cncb.ac.cn/gsa/ Experimental models: Cell lines Caco-2 ATCC Cat#HTB-37 Experimental models: Organisms/strains BALB/cNj-Foxn1nu/Gpt GemPharmatech D000521 RRID:IMSR_GPT:D000521 C57BL/6JGpt GemPharmatech N000013 RRID:IMSR_GPT:N000013 BALB/cJGpt GemPharmatech N000020 RRID:IMSR_GPT:N000020 C57BL/6JGpt-Rag2em6Cd6972/Gpt GemPharmatech T011534 RRID:IMSR_GPT:T011534 C57BL/6JGpt-Il22em4Cd16159/Gpt GemPharmatech T052618 RRID:IMSR_GPT:T052618 C57BL/6Smoc-Cd74em1Smoc Shanghai Model Organisms NM-KO-190772 RRID: IMSR_NM-KO-190772 Oligonucleotides Primers for qPCR, see Table S3 Sangon Biotech N/A (Continued on next page) e2 Cell Host & Microbe 34, 1–16.e1–e8, March 11, 2026

    Article Title: Commensal enteric virome regulates intestinal carbohydrate digestion and absorption.
    Article Snippet: .. A Mouse IL-22 High Sensitivity ELISA Kit (EK222HS, Multi sciences), IL-23 ELISA Kit (EK223, Multi sciences), insulin (MM-0579M2, MEIMIAN), IL-6 (R-S-M0007, RUISAIQI), IL-1β (R-S-M0003, RUISAIQI), TNF-α (R-S-M0002, RUISAIQI) and adiponectin (R-S-M10108, RUISAIQI) were utilized to measure the protein concentrations according to the manufacturer’s instructions. .. T cells were depleted using 250 μg of anti-CD4, anti-CD8 antibodies (BE0003-41, BE0004-1, BioXcell), while F4/80+ APCs were eliminated with clodronate liposomes (40337ES08, Yeasen) following the manufacturer’s instructions.

    Article Title: A simple two-dimensional metal–organic framework–based phototherapy nanoplatform with a triple-synergistic mechanism for enhanced wound infection treatment
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: Human umbilical cord-derived mesenchymal stem cells ameliorate non-alcoholic fatty liver disease via activating TFEB-mediated autophagy in male mice
    Article Snippet: ImageJ software (NIH) was used for densitometric analysis of the bands. .. The levels of IL-6 were measured using Mouse IL-6 High Sensitivity ELISA Kit according to the manufacturer’s instructions (Multi Sciences, EK206HS-96). .. Mouse IL-1β ELISA Kit (Multi Sciences, EK201B/3–96), Human IL-1β ELISA Kit (Multi Sciences, EK101B), and Human/Mouse/Rat TGF-β1 ELISA Kit (Multi Sciences, EK981-96) were used to detect the levels of IL-1β and TGF-β1 respectively.

    Article Title: Feasibility of combining JAK1 gene editing via CRISPR-CasRx with EGCG–lactoferrin nanoparticle therapy in a microneedle-based platform for atopic dermatitis
    Article Snippet: The mouse IgE ELISA Kit and mouse TSLP ELISA Kit were sourced from Yuanju Biotechnology Center (Shanghai, China). .. The 8-OHdG ELISA Kit was purchased from Youersheng Technology Co., Ltd (Wuhan, China), and the mouse IL-13 uncoated ELISA kit was purchased from Lianke Biotechnology Co., Ltd (Hangzhou, China). ..

    Article Title: Human umbilical cord-derived mesenchymal stem cells ameliorate non-alcoholic fatty liver disease via activating TFEB-mediated autophagy in male mice.
    Article Snippet: ImageJ software (NIH) was used for densitometric 244 analysis of the bands. .. 245 Enzyme-linked immunosorbent assay (ELISA) 246 The levels of IL-6 were measured using Mouse IL-6 High 247 Sensitivity ELISA Kit according to the manufacturer’s instructions 248 (Multi Sciences, EK206HS-96). .. Mouse IL-1β ELISA Kit (Multi 249 Sciences, EK201B/3-96), Human IL-1β ELISA Kit (Multi Sciences, 250 EK101B) and Human/Mouse/Rat TGF-β1 ELISA Kit (Multi Sciences, 251 EK981-96) were used to detect the levels of IL-1β and TGF-β1 252 respectively.

    Article Title: A Rationally Engineered Spleen‐Tropic One‐Component Lipid‐mRNA Complex (OncoLRC) for Cancer Vaccines
    Article Snippet: .. Cytokine levels in serum were quantified using commercial ELISA kits according to manufacturers' protocols: IFN‐α was measured using the ELISA MAX Deluxe Set Mouse IFN‐α kit (BioLegend, 447 904); IL‐12p40 was analyzed with the Mouse IL‐12/IL‐23p40 ELISA Kit (Lianke Bio, Hangzhou, EK2183); and IFN‐γ was assessed using the Mouse IFN‐γ ELISA Kit (Lianke Bio, Hangzhou, EK280HS). ..

    Article Title: A Rationally Engineered Spleen-Tropic One-Component Lipid-mRNA Complex (OncoLRC) for Cancer Vaccines.
    Article Snippet: .. Cytokine levels in serum were quantified using commercial ELISA kits according to manufacturers’ protocols: IFN-α wasmeasured using the ELISAMAXDeluxe SetMouse IFN-α kit (BioLegend, 447 904); IL-12p40 was analyzed with the Mouse IL-12/IL-23p40 ELISA Kit (Lianke Bio, Hangzhou, EK2183); and IFN-γ was assessed using the Mouse IFN-γ ELISA Kit (Lianke Bio, Hangzhou, EK280HS). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Commensal enteric virome regulates intestinal carbohydrate digestion and absorption.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CD45 Rabbit PolymAb ABclonal Cat#A24802PM CD3E Rabbit mAb ABclonal Cat#A19017 APC Rat Anti-Mouse CD8a(53–6.7) BD Pharmingen Cat#561093 PE-Cy7 Rat anti-Mouse CD4 Antibody(GK1.5) Absin Cat#abs1850076 PE-Cy7 Rat Anti-Mouse CD127(SB/199) BD Pharmingen Cat#560733 Alexa Fluor 647 anti-mouse IL-22 Biolegend Cat#516406 FITC Rat Anti-Mouse CD45 BD Pharmingen Cat#561088 PE Rat Anti-Mouse IL-17A BD Pharmingen Cat#561020 PerCP-CyTM5.5 Mouse Anti-Human CD3 BD Pharmingen Cat#560835 PerCP-CyTM5.5 Mouse Lineage Antibody Cocktail BD Pharmingen Cat#561317 PE Rabbit anti-Mouse T-bet/Tbx21 mAb ABclonal Cat#A28217 PE-CyTM7 Rat Anti-Mouse IFN-γ BD Pharmingen Cat#557649 SGLT1 Polyclonal antibody Proteintech Cat#30861-1-AP GLUT2 Polyclonal antibody Proteintech Cat#20436-1-AP InVivoMAb anti-mouse IL-23 (p19) BioXcell Cat#BE0313 IL-22 Monoclonal Antibody (IL22JOP) ThermoFisher Cat#16-7222-82 InVivoMAb anti-mouse MHC Class II (I-A/I-E) BioXcell Cat#BE0108 InVivoMAb anti-mouse CD4 (Clone: GK1.5) BioXcell Cat#BE0003-41 InVivoMAb anti-mouse CD8α (Clone: 53–6.7) BioXcell Cat#BE0004-1 APC Rabbit anti-Mouse F4/80 mAb ABclonal Cat#A28171 Bacterial and virus strains Streptococcus salivarius BNCC Cat#BNCC134928 Staphylococcus epidermidis BNCC Cat#BNCC102555 Pseudomonas aeruginosa BNCC Cat#BNCC340634 Clostridium butyricum BNCC Cat#BNCC337239 Escherichia coli C-3000 HZBMC Cat#HZB364542 Escherichia phage T7 BIOBW Cat#bio-108775 Primary enteric bacteriophages This study Table S2 Chemicals, peptides, and recombinant proteins Mouse IL-22 Recombinant Protein ThermoFisher Cat#210-22 IFN-lambda 3/IL-28B Protein, Mouse (HEK293, His) MedChemExpress Cat#HY-P74851 IL-17A Protein, Mouse (CHO) MedChemExpress Cat#HY-P7372 Acriflavine Aladdin Cat#A103028 Lamivudine Aladdin Cat#L408170 Aciclovir Aladdin Cat#A126073 Ribavirin Aladdin Cat#R101754 Ampicillin Aladdin Cat#A433389 Vancomycin Aladdin Cat#V301569 Neomycin sulfate Aladdin Cat#N109017 Metronidazole Aladdin Cat#M408417 Amphotericin B Aladdin Cat#A408132 PEG 8000 Sangon Biotech Cat#A100159 SYBR Gold nucleic acid gel stain ThermoFisher Cat#S11494 (Continued on next page) Cell Host & Microbe 34, 1–16.e1–e8, March 11, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER LB Broth hopebio Cat#HB0128 Nutrient Broth hopebio Cat#HB0108 Collagenase type 3 Worthington Cat#LS004182 Percoll Sigma Cat#P7828 Clodronate liposomes Yeasen Cat#40337ES08 Trizol ThermoFisher Cat#15596018CN DNase Sigma Cat#DN-25 Phenol/Chloroform/Isoamyl alcohol (25:24:1) Sigma Cat#P3803 Phusion DNA polymerase New England Biolabs Cat#M0530S 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester Aladdin Cat#C107884 Lipofectamine 2000 ThermoFisher Cat#11668030 Critical commercial assays Periodic Acid-Schiff Staining Kit Beyotime Cat#C0142S Pan T Cell Isolation Kit II, mouse Miltenyi Biotec Cat#130-095-130 Anti-F4/80 MicroBeads UltraPure, mouse Miltenyi Biotec Cat#130-110-443 EasySep Mouse Pan-ILC Enrichment Kit STEMCELL Technologies Cat#19875 Mouse IL-22 High Sensitivity ELISA Kit Multi sciences Cat#EK222HS Mouse IL-23 ELISA Kit Multi sciences CatEK223 Mouse Insulin ELISA Kit MEIMIAN Cat#MM-0579M2 Mouse IL-6 ELISA Kit RUISAIQI Cat#R-S-M0007 Mouse IL-1β ELISA Kit RUISAIQI Cat#R-S-M0003 Mouse TNF-α ELISA Kit RUISAIQI Cat#R-S-M0002 Mouse Adiponectin ELISA Kit RUISAIQI Cat#R-S-M10108 Evo M-MLV RT Kit with gDNA Clean for qPCR Accurate biology Cat#AG11728 TB Green Premix Ex Taq II (Tli RNase H Plus) Takara Cat#RR820Q E.Z.N.A. .. DNA Kit Omega Bio-tek Cat#D3892-01 TruSeq Nano DNA sample preparation Kit Illumina Cat#15041877 QIAamp MinElute Virus Spin Kit Qiagen Cat#57704 QIAquick Gel Extraction Kit Qiagen Cat#28706 TruSeq RNA Library Prep Kit Illumina Cat#RS-122-2001 Ribo-Zero rRNA Removal Kit Epicenter Cat#15066012 BCA Protein Assay Kit ThermoFisher Cat#223250 quick-dna fecal/soil microbe miniprep kit Zymo research Cat#D6010S Deposited data Enteric virome and bacteriome sequencing, and mouse small intestinal scRNA-seq and bulk RNA sequencing This study PRJCA029200 https://ngdc.cncb.ac.cn/gsa/ Experimental models: Cell lines Caco-2 ATCC Cat#HTB-37 Experimental models: Organisms/strains BALB/cNj-Foxn1nu/Gpt GemPharmatech D000521 RRID:IMSR_GPT:D000521 C57BL/6JGpt GemPharmatech N000013 RRID:IMSR_GPT:N000013 BALB/cJGpt GemPharmatech N000020 RRID:IMSR_GPT:N000020 C57BL/6JGpt-Rag2em6Cd6972/Gpt GemPharmatech T011534 RRID:IMSR_GPT:T011534 C57BL/6JGpt-Il22em4Cd16159/Gpt GemPharmatech T052618 RRID:IMSR_GPT:T052618 C57BL/6Smoc-Cd74em1Smoc Shanghai Model Organisms NM-KO-190772 RRID: IMSR_NM-KO-190772 Oligonucleotides Primers for qPCR, see Table S3 Sangon Biotech N/A (Continued on next page) e2 Cell Host & Microbe 34, 1–16.e1–e8, March 11, 2026



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    Journal: Bioactive Materials

    Article Title: Dynamic feedback BacGuard anchors microbial metabolism to host symbiosis in real-time ulcerative colitis therapy

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    Figure Lengend Snippet: BacGuard promotes colon tissue repairment. a) Scheme of microbiota-dependent epithelial repair mechanism orchestrated by BacGuard. b) Short-chain fatty acid (SCFA) profile alterations. n = 6. c) BacGuard-induced probiotic proliferation and d) quantitative results. n = 3. e) Immunofluorescence analysis of ILC3 (ROR γt + CD3 − cells) in colon tissue. f) Flow cytometric analysis of lamina propria lymphocytes (ROR γt + ). n = 3. g) Concentration of IL-22 in MNK-3 cells. n = 3. h) Representative PAS-staining (upper panel) and MUC-2 immunohistochemistry (lower panel) images of colon tissues. n = 5. ns, not significant; ∗p < 0.05, ∗∗p < 0.01 and ∗∗∗p < 0.001.

    Article Snippet: For the detection of IL-22 and LPS concentrations, the Mouse IL-22 Precoated ELISA Kit (DAKEWE, China) and Mouse LPS ELISA Kit (JONLNBIO, China) were employed correspondingly.

    Techniques: Immunofluorescence, Concentration Assay, Staining, Immunohistochemistry